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epr [2019-03-13 17:15:36]
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epr [2019-03-13 17:15:36]

minor extracellular serine protease, involved in control of swarming motility
locus
BSU38400
pI
9.88
mw
69.52 kDa
protein length
645 aa Sequence Blast
gene length
1938 bp Sequence Blast
function
protein degradation
product
minor extracellular serine protease
essential
no
synonyms
ipa-15r
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    3,939,869 → 3,941,806

    The protein

    Protein family

  • [SW|peptidase S8 family] (according to UniProt)

    • Structure

  • [PDB|3WHI] ([protein|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|subtilisin], corresponds to aa 27 ... 382, 40% identity)

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|F2E3FF41357C672F80012CFE3076F890407B8B4A|epr]
    description
    [Pubmed|3142851]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|11751842], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16923912], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|16923912], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, not activated by [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P [Pubmed|19416356], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

    • regulation

  • repressed by [protein|search|SinR] [Pubmed|16923912]
    • view in new tab

    Biological materials

    Mutant

  • 1A1021 ( ''epr''::''tet''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1021&Search=1A1021 BGSC]
  • KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133
  • BKE38400 (Δ[gene|F2E3FF41357C672F80012CFE3076F890407B8B4A|epr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCATCTCCTTTTTC, downstream forward: _UP4_TAACCAAAAACCTTTAAGAT
  • BKK38400 (Δ[gene|F2E3FF41357C672F80012CFE3076F890407B8B4A|epr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCATCTCCTTTTTC, downstream forward: _UP4_TAACCAAAAACCTTTAAGAT

    • References

    Reviews

  • 20735481

    • Original publications

  • 16923912,3142851,19416356,18957862,19416356,11751842,23660663,24115457